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Infectivity of Zika Virus on Primary Cells Support Tree Shrew as Animal Model
论文题目: Infectivity of Zika Virus on Primary Cells Support Tree Shrew as Animal Model
作者: Zhang L, Shen ZL, Feng Y, Li DQ, Zhang NN, Deng YQ, Qi XP, Sun XM, Dai JJ, Yang CG, Yang ZF, Qin CF, Xia XS
联系作者: qincf@bmi.ac.cn
发表年度: 2019
DOI: doi: 10.1080/22221751.2018.1559707
摘要:

Zika virus (ZIKV) is a mosquito-borne flavivirus that caused the public health emergency. Recently, we have proved a novel small animal treeshrew was susceptive to ZIKV infection and presented the most common rash symptoms as ZIKV patients. Here we further cultured the primary cells from different tissues of this animal to determine the tissue tropism of ZIKV infection in vitro. The results showed that the primary cells from tree shrew kidney, lung, liver, skin and aorta were permissive to ZIKV infection and could support viral replication by the detection of viral specific RNA intra- and extra-cells. In comparing, the skin fibroblast and vascular endothelial cells were highly permissive to ZIKV infection with high releasing of active virus particles in supernatants proved by its infectivity in established neonatal mouse model. The expressions of ZIKV envelop and nonstructural protein-1, and the effects and strong immune response of primary tree shrew cells were also detected followed by ZIKV infection. These findings provide powerful in vitro cell-level evidence to support tree shrew as animal model of ZIKV infection and may help to explain the rash manifestations in vivo

刊物名称: Emerging Microbes & Infections
论文出处: https://www.tandfonline.com/doi/full/10.1080/22221751.2018.1559707
影响因子: 6.212(2019年)
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